Potato is an essential crop in Bali. The potato productivity in Bali is still low compared to other provinces in Indonesia. The main problem is difficulties in obtaining quality seeds at affordable prices. This study aimed to ensure the survival of tissue culture seedlings during acclimatization and produce good quality early-generation seed potatoes. The research was conducted at Batusesa Village, Tabanan, Bali, from March - July 2021. There were two experiments, the first was the production of G0 seed potato, and the second was the production of G1 seed. Production of G0 was done using Factorial Randomized Block Design with two factors, variety (Granola and Vega) and media (charred rice husk, charredrice husk + coco peat, charred rice husk + coco peat + soil). Each treatment combination consisted of 100 cuttings. Variables observed were harvest time, tuber weight, and total tubers per plant. The result shows that Vega has a shorter harvesting time (65 days) compared to Granola (70 days). Vega produced heavier tuber in all media trials. Granola made more tubers in M3 media. Experiment on G1 production was done using Randomized Block Design with two factors, variety (Granola and Vega) and G0 seed tuber size (<15 gram and ≥ 15 gram; <50 gram and ≥ 50 gram). Vega produced more tubers than Granola; while Granola produced bigger tubers than Vega. Bigger seed tubers had the highest tuber production and bigger size tubers in both varieties.
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