Streptomyces macrolidinus sp. nov., a novel soil actinobacterium with potential anticancer and antimalarial activity

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A novel actinomycete, strain RY43-2T, belonging to the genus Streptomyces, was isolated from a peat swamp forest soil collected from Rayong Province, Thailand. The strain was characterized by using a polyphasic approach. The cell-wall peptidoglycan contained ll-diaminopimelic. Ribose and glucose were detected in its whole-cell hydrolysates. The strain contained anteiso-C15:0, iso-C14:0 and iso-C16:0 as the predominant fatty acids, and MK-9(H4), MK-9(H6) and MK-9(H8) as the major menaquinones. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, three unidentified ninhydrin-positive phospholipids and two unidentified phospholipids. Strain RY43-2T showed the highest 16S rRNA gene similarity to Streptomyces misionensis JCM 4497T (98.9%) and Streptomyces lichenis LCR6-01T (98.9%). The draft genome of RY43-2T was 6.7 Mb with 6078 coding sequences with an average G+C content of 70.8 mol%. Genomic analysis revealed that the average nucleotide identity (ANI) values based on blast (ANIb) and MUMmer (ANIm) between strain RY43-2T and S. misionensis JCM 4497T were 80.1 and 86.1%, respectively. The ANIb and ANIm values between strain RY43-2T and S. lichenis LCR6-01T were 77.0 and 85.5%, respectively. The digital DNA–DNA hybridization values were 25.2 and 23.0% in comparison with the draft genomes of S. misionensis JCM 4497T and S. lichenis LCR6-01T, respectively. The results of taxonomic analysis suggested that strain RY43-2T represented a novel species of the genus Streptomyces for which the name Streptomyces macrolidinus sp. nov. is proposed. The type strain is RY43-2T (=TBRC 7286T=NBRC 115640T). Strain RY43-2T exhibited antimicrobial activity against Enterococcus faecium ATCC 51559, Colletotrichum capsici BMGC 106 and Colletotrichum gloeosporioides BMGC 107 with the minimum inhibitory concentration values of 25.0, 12.5, and 6.25 µg ml?1. It also exhibited potent antimalarial activity against Plasmodium falciparum K1 with IC50 of 0.0031 µg ml?1. In addition, it showed cytotoxicity against Vero, KB, MCF-7 and NCI-H187 with IC50 values of 0.0347, 6.15, 3.36 and 0.0352 µg ml?1, respectively. © 2023 The Authors. View source


Secondary Title

International Journal of Systematic and Evolutionary Microbiology


Microbiology Society









actinomycetota; anticancer; antimalarial; peat swamp forest; Streptomyces macrolidinus; antimalarial agent; antineoplastic agent; bacterial DNA; bacterial extract; bacterial RNA; cardiolipin; fatty acid; genomic DNA; glucose; mannoside; menaquinone; ninhydrin; nucleotide; peptidoglycan; phosphatidylethanolamine; phosphatidylglycerol; phosphatidylinositol; phospholipid; ribose; RNA 16S; Streptomyces macrolidinus extract; unclassified drug; antimalarial agent; bacterial DNA; diaminopimelic acid; RNA 16S; Acinetobacter baumannii; Alternaria brassicicola; animal cell; antimalarial activity; antineoplastic activity; Article; Bacillus cereus; bacterial cell wall; bacterial genome; bacterial strain; bacterium isolate; bacterium isolation; Candida albicans; chemotaxonomy; Colletotrichum capsici; Curvularia lunata; cytotoxicity; DNA base composition; DNA DNA hybridization; drug isolation; Enterococcus faecium; Escherichia coli; forest soil; genomics; Glomerella cingulata; human; human cell; IC50; KB cell line; Klebsiella pneumoniae; Magnaporthe grisea; minimum inhibitory concentration; molecular phylogeny; morphological trait; NCI-H187 cell line; nonhuman; nucleotide sequence; phylogenetic tree; Plasmodium falciparum; Pseudomonas aeruginosa; soil microflora; Streptomyces; Streptomyces lichenis; Streptomyces macrolidinus; Streptomyces misionensis; swamp; Thailand; type strain; Vero cell line; whole cell; whole genome sequencing; bacterium identification; chemistry; DNA sequence; genetics; phylogeny; soil; Antimalarials; Bacterial Typing Techniques; Base Composition; Diaminopimelic Acid; DNA, Bacterial; Fatty Acids; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil; Streptomyces; Thailand

Form: Journal Article
Geographical Area: Thailand

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